Stem Cell Feeder Cells

Mouse Embryo Fibroblasts (MEF) and Newborn Human Foreskin Fibroblasts (NuFF)

The quality of feeder cells is a critical factor in maintaining stem cell pluripotency. Lot-to-lot consistency must be tightly controlled through well-established, robust manufacturing practices. At MTI-GlobalStem, each lot of feeder cells is thoroughly PluriQ™ tested for the highest quality assurance. This includes comprehensive testing on mouse and/or human ES cells to ensure robust and consistent performance with every lot.  Our human fibroblasts (NuFF) and mouse embryonic fibroblasts (MEF) are available in a variety of configurations, to ensure successful culture conditions in your laboratory. Click on the specific configuration of interest below for more details.

human newborn fibroblasts and CF1 mouse embryonic fibroblasts

Mouse Feeder Cells (MEF)

We offer mouse embryo fibroblast (MEF) cells as untreated and pre-treated (i.e. mitotically arrested). In order to use mouse embryo fibroblast (MEF) cells as a feeder layer the cells have to be mitotically arrested to keep them from outgrowing the cells they are supporting. This treatment, whether by irradiation or chemical exposure, limits the culture life of the feeders. Our irradiated (IRR) and Mitomycin C-treated (Mito-C) MEF optimally support the embryonic stem (ES) cells for 7-10 days. After this time, ES cells are generally plated on a new feeder layer each time they are expanded to new plates/dishes. Mouse ES usually require passage every 2 days and human ES every 4-5 days. We recommend plating our MEF in DMEM+FBS and then changing the media to your DMEM/F12 formulation when you plate your cells. Allow the MEF 24 hours to establish a good feeder layer before adding your stem cells. Our MEF do not require gelatin coating of plates prior to use.

Product Description
CF-1 MEF IRR
Traditional mouse embryonic fibroblast feeder cells for culturing pluripotent stem cells; Mitotically arrested by Irradiation and will not proliferate.
CF-1 MEF Mito-C
Traditional mouse embryonic fibroblast feeder cells for culturing pluripotent stem cells; Mitotically arrested by Mitomycin-C and will not proliferate.
CF-1 MEF
Traditional mouse embryonic fibroblasts for culturing pluripotent stem cells; Untreated and will proliferate.
C57BL/6 MEF IRR
Irradiated mouse embryonic fibroblast feeder cells to support and maintain ESC/iPSC in the undifferentiated state; will not proliferate.
C57BL/6 MEF Mito-C
Mitomycin-C treated mouse embryonic fibroblast feeder cells to support and maintain ESC/iPSC in the undifferentiated state; will not proliferate.
C57BL/6 MEF
Untreated mouse embryonic fibroblasts to support and maintain ESC/iPSC in the undifferentiated state; cells will proliferate.
Puro-MEF IRR
Irradiated puromycin-resistant mouse embryonic fibroblast feeder cells; will not proliferate.
Puro-MEF Mito-C
Mitomycin-C treated puromycin-resistant mouse embryonic fibroblast feeder cells; will not proliferate.
CF6 Neo MEF IRR
Irradiated neomycin-resistant mouse embryonic fibroblast feeder cells; will not proliferate.
CF6 Neo MEF Mito-C
Mitomycin-C treated neomycin-resistant mouse embryonic fibroblast feeder cells; will not proliferate.
CF6 Neo MEF
Untreated neomycin-resistance mouse embryonic fibroblasts; cells will proliferate.
DR4 MEF
Irradiated puromycin, hygromycin, puromycin, and 6-thioguanine drug resistant mouse embryonic fibroblast feeders; will not proliferate.

Swiss Webster-mef

Irradiated Swiss-Webster mouse stain feeders used to support healthy undifferentiated human and mouse ES in culture; will not proliferate.

Human Feeder Cells (NuFF)

Product Treatment / Recommended Use
(Reprogramming-qualified) Irradiated; recommended as feeder cells during iPSC reprogramming ; See also NuFF-RQ3™(Reprogramming-qualified fibroblast control cells for iPSC-generation)
NuFF IRR
Irradiated newborn human foreskin fibroblasts used as feeder cells for pluripotent stem cell culture
NuFF Mito-C
Mitomycin-C treated newborn human foreskin fibroblasts used as feeder cells for pluripotent stem cell culture
NuFF
Untreated newborn human foreskin fibroblasts used as feeder cells for pluripotent stem cell culture