Short Tandem Repeats (STR)
STR are short, repetitive DNA sequences 3 to 7 base pairs in length. The number of repeats at a particular locus can vary from one individual to the next and can be used to discriminate between individuals. These repeats are well distributed throughout the human genome and are a rich source of highly polymorphic markers, which are routinely detected using PCR. These loci satisfy the needs of several major standardization bodies throughout the world – e.g. the US FBI has selected 13 STR core loci from the set of 16 to search or include samples in CODIS (COmbined DNA Index System), the U.S. national database of convicted offender profiles. Many laboratories routinely perform STR on their ESC lines as early as possible to establish the identity of that line. STR is periodically re-run and checked against the initial reference pattern. A sample STR 8 loci electro-pherogram from the BG01 hESC line is shown in Figure 1 (below).
Figure 1. STR electropherogram of the BG01 hESC line. Eight STR loci were examined (D5S818, D13S317, D16S539, vWa, TH01, TP0X, CSF1P0, and amelogenin) to produce this unique DNA fingerprint.
Short Tandem Repeat (STR) Analysis is critical for verifying the identity of human cell lines, ensuring uniqueness of the cell line and detecting laboratory errors such as misidentification and cross-contamination of lines. An STR profile should be made for all newly derived cell lines. This allows for a periodic check of the STR profiles against the original baseline profile to ensure the continued integrity of the cells and your experimental data.
Short Tandem Repeat (STR) Profile
- Product Code: GSS-3002
- Availability: In Stock