Transfection protocols rely on reagents that safely deliver nucleic acids with maximal efficiency and effectiveness, while minimizing cellular toxicity. Transfection reagents are not one-size-fits-all; rather, reagents are often selective in the types of cells and nucleic acids with which they work best.
MTI-GlobalStem has formulated a family of DNA and mRNA transfection reagents that balance high transfection and expression levels with low toxicity across a range of common passage cells lines and primary cells. Developed by the lead members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000, our transfection reagents are formulations of proprietary compounds and are chemically defined and of animal-free origin. They are routinely tested on a wide range of cell lines and designed to require minimal optimization to achieve superior gene-delivery and expression results.
- General-Purpose Transfection
- Cell-Specific DNA Transfection
- Cell-Specific mRNA Transfection
- Genome Editing Transfection
|Transfection Application||Product Name||Substrate Delivery||Description|
|CRISPR/Cas9 Plasmid Delivery||DNA-In® CRISPR||DNA|
Formulated for large plasmid delivery; Optimized for intracellular transfection of CRISPR/Cas9 all-in-one vectors into primary cells and other cell types for Genome Editing applications.
| NEW Genome Editing in Stem Cells||EditPro™ Stem||DNA/RNA/protein||Optimized for delivery of DNA, RNA, and/or protein. Ideal for Genome Editing via CRISPR/Cas9, TALENs or Zinc Fingers. Best used as part of complete PluriQ™ G9™ Editing System.|
|NEW Genome Editing in Adherent Cells||EditPro™|| Cas9 mRNA |
|Optimized for the delivery of Cas9 mRNA or protein (RNP) and related guides for CRISPR gene editing in a range of adherent primary cells and cell lines.|
|Type||Product Name||Substrate Delivery||Recommended Application (s)|
|General-Purpose Reagents||DNA-In®||DNA||Passaged cell lines (e.g. NIH-3T3, HeLa, MCF7, etc,) and most primary cells.|
|Common Cell Lines||DNA-In® A549||DNA||Optimized specifically for A549 (adenocarcinomic human alveolar basal epithelial) cells|
|DNA-In® CHO||DNA||Optimized for adherent CHO (Chinese Hamster Ovary)cells|
|DNA-In® 293||DNA||Optimized for HEK 293 (Human Embryonic Kidney)cells|
|DNA-In® HepG2||DNA||Optimized for HepG2 (human hepatocellular carcinoma) cells|
|DNA-In® Jurkat New||DNA||Optimized for Jurkat (human T lymphocyte line) cells|
|DNA-In® SY5Y||DNA||Optimized specifically for SH-SY5Y (neuroblastoma) cells|
|Specialty Cells||DNA-In® Neuro||DNA||Formulated & optimized specifically for neurons|
|DNA-In® Stem||DNA||Formulated & optimized specifically for hard-to-transfect stem cells, including neural stem cells|