Transfection Reagents

Transfection protocols rely on reagents that safely deliver nucleic acids with maximal efficiency and effectiveness, while minimizing cellular toxicity. Transfection reagents are not one-size-fits-all; rather, reagents are often selective in the types of cells and nucleic acids with which they work best.

Tubes of transfection reagent

MTI-GlobalStem has formulated a family of DNA and mRNA transfection reagents that balance high transfection and expression levels with low toxicity across a range of common passage cells lines and primary cells. Developed by the lead members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000, our transfection reagents are formulations of proprietary compounds and are chemically defined and of animal-free origin. They are routinely tested on a wide range of cell lines and designed to require minimal optimization to achieve superior gene-delivery and expression results.

Below is a guide to the appropriate reagent based on substrate, cell type, and/or application. Should you have questions, please email us and we are happy to help.


Transfection ApplicationProduct NameSubstrate DeliveryDescription
 CRISPR/Cas9  Plasmid Delivery DNA-In® CRISPRDNA

 Formulated for large plasmid delivery; Optimized for intracellular transfection of  CRISPR/Cas9 all-in-one vectors into primary cells and other cell types for Genome  Editing applications.

Genome Editing in  Stem Cells
Invitrogen™ Lipfectamine™ Stem Transfection Reagent
exclusively via thermofisher.com
  Cas9 plasmid
Cas9 mRNA 
Cas9 protein
ssDNA/ssODN
 Optimized for delivery of DNA, RNA, and/or RNP. Ideal for Genome Editing via  CRISPR/Cas9, TALENs or Zinc Fingers. 
 NEW                         Genome Editing in  Adherent Cells EditPro™    Cas9 mRNA 
Cas9 protein
ssDNA/ssODN 
 Optimized  for the delivery of Cas9 mRNA or protein (RNP)  and related guides for CRISPR gene editing in a range of adherent primary cells and cell lines.

TypeProduct NameSubstrate DeliveryRecommended Application (s)
 General-Purpose  Reagents DNA-In®DNA  Passaged cell lines (e.g. NIH-3T3, HeLa, MCF7, etc,) and most primary  cells.
 mRNA-In®mRNA
 Cell-Specific Reagents
 Common Cell Lines DNA-In® A549DNA Optimized specifically for A549 (adenocarcinomic human alveolar basal  epithelial) cells
 DNA-In® CHODNA Optimized for adherent CHO (Chinese Hamster Ovary)cells
 DNA-In® 293DNA Optimized for HEK 293 (Human Embryonic Kidney)cells
 DNA-In® HepG2DNA Optimized for HepG2 (human hepatocellular carcinoma) cells
 DNA-In® Jurkat        New DNA Optimized for Jurkat (human T lymphocyte line) cells
 DNA-In® SY5YDNA Optimized specifically for SH-SY5Y (neuroblastoma) cells
 Specialty Cells DNA-In® NeuroDNA Formulated & optimized specifically for neurons
 mRNA-In® NeuromRNA
Invitrogen™ Lipfectamine™ Stem Transfection Reagent
exclusively via thermofisher.com
DNA
mRNA
 Formulated & optimized specifically for hard-to-transfect stem cells,  including neural stem cells
DNA-In® & mRNA-In®are registered trademarks of Molecular Transfer, Inc. (dba MTI-GlobalStem)
Lipofectamine™ is a registered trademark of Thermo Fisher Scientific Inc.


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DNA-In® CRISPR Transfection Reagent

DNA-In® CRISPR Transfection Reagent

NEW DNA-In® CRISPR Transfection Reagent   Specifically formulated for CRISPR/Cas9 trans..

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EditPro™  Transfection Reagent

EditPro™ Transfection Reagent

EditPro™  Transfection Reagent has been optimized to deliver Cas9 mRNA or RNP with the associat..

$257.50

EditPro™ Stem  Transfection Reagent has been replaced by Lipofectamine™ Stem Transfection Reagent

EditPro™ Stem Transfection Reagent has been replaced by Lipofectamine™ Stem Transfection Reagent

EditPro™ Stem Transfection Reagent is optimized for genome editing in human pluripotent stem cells a..

$257.50

mRNA-In® Neuro Transfection Reagent

mRNA-In® Neuro Transfection Reagent

mRNA-In® Neuro Messenger RNA Delivery into Neurons  mRNA-In® Neuro is a new mRNA..

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DNA-In® Transfection Reagent

DNA-In® Transfection Reagent

DNA-In® Transfection Reagent A Universal Transfection Reagent for DNA Delivery DNA-In® &nbs..

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mRNA-In®  Transfection Reagent

mRNA-In® Transfection Reagent

mRNA-In® A Universal Transfection Reagent for High Efficiency mRNA DeliverymRNA-In® Transfectio..

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DNA-In® 293 Transfection Reagent

DNA-In® 293 Transfection Reagent

DNA-In® 293 Transfection Reagent A Transfection Reagent Specifically for HEK293 Cells  DNA-In..

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DNA-In® A549 Transfection Reagent

DNA-In® A549 Transfection Reagent

DNA-In® A549 Transfection Reagent A Transfection Reagent for A549 Cells  DNA-In® Ce..

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DNA-In® CHO Transfection Reagent

DNA-In® CHO Transfection Reagent

DNA-In® CHO Transfection Reagent Formulated Specifically for CHO-K1 Cells​  DNA-In®..

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DNA-In® HepG2 Transfection Reagent

DNA-In® HepG2 Transfection Reagent

DNA-In® HepG2  Formulated Specifically for HepG2 Cells  DNA-In® Cel..

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DNA-In® Neuro Transfection Reagent

DNA-In® Neuro Transfection Reagent

DNA-In® Neuro Transfection ReagentFormulated Specifically for Neurons Developed by th..

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DNA-In® Stem Transfection Reagent has been replaced with Lipofectamine Stem Transfection Reagent

DNA-In® Stem Transfection Reagent has been replaced with Lipofectamine Stem Transfection Reagent

DNA-In® Stem Transfection ReagentOptimized for maximum DNA delivery in Stem Cells The..

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DNA-In® SY5Y Transfection Reagent

DNA-In® SY5Y Transfection Reagent

DNA-In® SY5Y Transfection ReagentOptimized for SH-SY5Y CellsSH-SY5Y  is a human derived neurobl..

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