A new basal medium formulated for maximum growth and survival of proliferative neural cells such as human iPSC-derived neural stem cells or rat primary oligodendrocyte precursors. It is intended for use with GS22™ Neural Supplement (GSM-3200). When combined to form a complete medium, NeuralX™ and GS22™ demonstrate superior performance in supporting the growth and survival of proliferating neural stem cells in culture.
- Improves long‐term viability of proliferative neural cells.
- Supports more efficient and higher yields of oligodendrocyte precursors.
- Serum-free, animal-free for minimal risk of introducing adventitious agents into the cell.
Supports Healthy Neural Stem Cells in Culture
Figure 1. NeuralX™ Neural Stem Cell Medium and GS22™ Neural Supplement support healthy growth of proliferating human iPSC-derived neural stem cells in culture over multiple passages. HIP™ Human iPSC-derived pluripotent neural stem cells (BC1 line) were maintained in NeuralX™ NSC Medium and GS22™ supplement for 4 days before passaging and continuing culture for another 4 days.
Optimal Differentiation into Healthy, Mature Oligodendrocytes
Figure 2. Rat primary oligodendrocyte precursors (GRPs) exhibit excellent growth, survival and health when grown in NeuralX™ NSC Medium and GS22™ Neural Supplement. After rat GRPs were grown for 3 passages in NeuralX™ NSC Medium with GS22™ supplement, antibody labeling indicated the cells were still primarily undifferentiated GRPs, as indicated by labeling with A2B5 (green) and DAPI (blue).
Supports Growth &Long-term Survival in Oligodendrocyte Precursors
Figure 3. Rat primary oligodendrocyte precursors (GRPs) differentiate into healthy, mature oligodendrocytes. After growing rat GRPs in in NeuralX™NSC Medium with GS22™ Supplement for 3 passages, cells were switched to NeuralQ™ Basal Medium supplemented with GS21™ Neural Supplement (GSM-3100) for 2 passages. Antibody labeling revealed a significant number of mature, differentiated oligodendrocytes, as indicated by labeling with GalC (green, left panel) and MBP (green, right panel). Total cells are shown using DAPI (blue, both panels
- Barratt, H. E., Budnick, H. C., Parra, R., Lolley, R. J., Perry, C. N., & Nesic, O. (2016). Tamoxifen promotes differentiation of oligodendrocyte progenitors in vitro. Neuroscience.
|Recommended Use||Growth and survival of neural stem cells and other proliferative neural cells|
|Product Unit||Bottle, sterile-filtered|
|Storage||Refrigerate at 4°C|
|Quality Testing||Sterility, bacterial and fungal testing|
|Other||Not intended for human or animal diagnostic or therapeutic uses|
NeuralX™ NSC Medium (1X)
- Product Code: GSM-9320
- Availability: In Stock