A Neuronal Basal Medium for Maximum Growth
A serum-free basal medium optimized for maximum growth and survival of primary and iPSC-derived neural cells in culture, and based on the previously published formulation by Brewer et al.1 Intended to be supplemented with the serum-free GS21™ Neural Supplement (GSM-3100) along with other standard neuronal growth factor supplements, NeuralQ™ Basal Medium shows superior performance in the overall cell health and viability of neurons during short and long-term culture.
Supports Healthy Primary Neurons in Culture
Image 1. Healthy, maturing primary rat neurons in culture - NeuralQ® Basal Medium supports maximum viability and healthy of primary rat neurons during short and longer-term culture.
Image 2. NeuralQ® supports development of mature neurons - Above images of primary rat neurons (Map2ab, green) cultured 15 days in NeuralQ® Basal Medium shows healthy, mature neurons in culture.
Outperforms Neurobasal® Medium in Long-Term Cultures
Image 3. NeuralQ® outperforms Neurobasal® Medium - Above images of primary rat neurons (Map2ab, green) were cultured 3 weeks in NeuralQ® Basal Medium (panel A) or Neurobasal (panel B) and supplemented with GS21™ Neural Supplement. The above data shows NeuralQ® Basal Medium significantly outperforms Neurobasal in supporting neurons growth (green) during long term culture. GFAP staining (red) and Hoechst (blue) label astrocytes and nuclei, respectively.
Significantly Better Support of Primary Mouse Cortical Neurons
Reference: Brewer, G.J., Torricelli, J.R., Evege, E.K. and Price, P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal™, A new serum-free medium combination. J. Neurosci. Res.35: 567-576 (1993).
- Tukker A.M., de Groot M.W., Wijnolts F.M., Kasteel E.E., Hondebrink L., Westerink R.H. (2016) Is the time right for in vitro neurotoxicitytesting using human iPSC-derived neurons? ALTEX
- Barratt, H. E., Budnick, H. C., Parra, R., Lolley, R. J., Perry, C. N., & Nesic, O. (2016). Tamoxifen promotes differentiation of oligodendrocyte progenitors in vitro. Neuroscience.
- Huang, J. Y., Wang, K., Vermehren-Schmaedick, A., Adelman, J. P., & Cohen, M. S. (2016). PARP6 is a Regulator of Hippocampal Dendritic Morphogenesis. Scientific reports, 6.
- Pellett, S., Schwartz, M. P., Tepp, W. H., Josephson, R., Scherf, J. M., Pier, C. L., ... & Johnson, E. A. (2015). Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin. Scientific reports, 5.
NeuralQ & GS21 are registered Molecular Transfer, Inc trademark products
|Recommended Use||Basal medium for maximum growth and survival of primary and iPS-derived neurons in culture.|
|Product Unit||Bottle, sterile-filtered|
|Storage||Refrigerate at 4°C|
|Quality Testing||Sterility, bacterial and fungal testing|
|Other||Not intended for human or animal diagnostic or therapeutic uses|
NeuralQ® Basal Medium (1X)
- Product Code: GSM-9420
- Availability: In Stock