• Human Fibroblasts

NuFF: Newborn Human Foreskin Fibroblasts

The success of maintaining human ESC and iPSC begins with propagating healthy feeder cultures.  Our human newborn fibroblasts (NuFF)are  long lifespan cells and idea as feeder cell support of  pluripotent stem cells in culture.   NuFF feeder cells support and maintain human ESC and iPSC in the undifferentiated state.  Untreated NuFF cells are mitotically and metabolically active. For mitotically inactive NuFF , use NuFF-IRR or NuFF Mito-C. 

  • Metabolically, Mitotically Active – Feeder cells are untreated for your specific experimental needs.
  • Consistent Performance – Our standardized manufacturing process yields low passage cells which consistently maintain ES and iPS cell cultures in an undifferentiated state.
  • Safe Handling – Our comprehensive contamination tests include human pathogen and Mycoplasma detection, keeping your lab safe from contamination. 

Meticulous Quality Control

Work with confidence. Comprehensive testing of each lot minimizes contamination risks to your lab. Our NuFF are derived and comprehensively tested on human ES cells to ensure robust and consistent performance with every lot. In addition they are:

  • Negative for bacterial/fungal contamination
  • Negative for MycoplasmaPCR assay
  • Negative for human pathogens
  • Post thaw viability: typically greater than 95%

Recommended Plating

The recommended seeding density is 2 x 104 to 3 x 104 cells/cm2; however, please follow the culture instructions provided with the specific ESC or iPSC lines that you are using. These cells should be plated 24 hours prior to plating the ES cells and can be used for 7–10 days. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the MEF are too dense, the feeder layer may detach from the plate, and the culture will be lost.

Save Time and Money

Save time and trouble dealing with an animal facility, performing tedious and time-consuming dissections and the time required to expand and test the cells. Day after day – week after week. Focus on your research.

Safe handling

Work with confidence. Our comprehensive contamination tests include human pathogen and Mycoplasma detection. This significantly minimizes the threat of contamination in your lab allowing you to work with greater confidence.

Technical Data

Publications

Information
Recommended Use Maintenance of undifferentiated ESC and iPSC lines
Cell Source Human Foreskin
Product Unit Cryopreserved Vial
Size 4-5 million cells/vial
Treatment Untreated
Storage Vapor phase of liquid nitrogen
Quality Testing Tested for high efficient maintenance of human pluripotent cells, sterility, human pathogens, mycoplasma, post thaw viability
Shipping Dry Ice
Passage Number P9
Other Donor 12

Human Fibroblasts 4M Untreated D12

  • Product Code: GSC-3002
  • Availability: In Stock
  • $181.44


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Tags: human, fibroblasts, untreated, d12, stem, cell, feeder, cells