Supporting iPS reprogramming
NuFF-RQ™ Human Fibroblasts are iPSC Reprogramming Qualified feeder cells ideal for supporting cells during mRNA reprogramming. During cellular reprogramming the feeder layer can greatly affect the health of the cell culture and the success of the reprogramming. While a range of mitotically inactivated fibroblasts can be used as a support layer for pluripotent stem cell culture, not all will support mRNA reprogramming. MTI-GlobalStem offers Newborn Human Foreskin Fibroblasts (NuFF-RQ™) that have been functionally validated to support the generation of induced pluripotent stem cell (iPSC) colonies using the Stemgent® mRNA Reprogramming Kit.
Key Features & Benefits
- Pre‐Validated for iPSC Reprogramming ‐ provides optimal feeder layer support during mRNA reprogramming.
- High Quality, Low Passage ‐ PluriQ™ tested fibroblasts provide consistent, long‐term support of healthy induced pluripotent stem cell cultures.
- Meticulous Quality Control – Comprehensive testing of each lot with human ES cells to ensure safety and performance.
Feeder Cells for Cellular Reprogramming
Reprogramming ValidationNuFF-RQ™ are tested for viability, attachment, and mitotic arrest (lack of expansion) during mRNA reprogramming. These cells have been confirmed to remain healthy and viable, providing quality layer support during repeat transfections of target cells during the mRNA reprogramming process.
Meticulous Quality ControlWork with confidence. Comprehensive testing of each lot minimizes contamination risks to your lab. Our human fibroblasts are derived and comprehensively tested on human ES cells to ensure robust and consistent performance with every lot. In addition they are:
- Negative for bacterial/fungal contamination
- Negative for Mycoplasma (via PCR analysis)
- Negative for human pathogens
- Typically greater than 95% viable post-thaw
- [PDF] Kent, L. T., & Ashley, R. Generation of Safe, Integration-free Human Induced Pluripotent Stem (iPS) Cells using the mRNA Reprogramming System.
- Mandal, Pankaj K., and Derrick J. Rossi. "Reprogramming human fibroblasts to pluripotency using modified mRNA." Nature protocols 8.3 (2013): 568-582.
- Warren, L., Manos, P.D., Ahfeldt, T., Loh, Y.H., Li, H., Lau, F., Ebina, W., Mandal, P.K., Smith, Z.D., Meissner, A., Daley, D.Q., Brack, A.S., Collins, J.J., Cowan, C.,Schlaeger, T.M., and Rossi, D.J. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7: 618-630.
- Warren L and Wang J (2013) Feeder‐Free Reprogramming of Human Fibroblasts with Messenger RNA, Current Protocols in Stem Cell Biology. 27:4A.6.1–4A.6.27.
- Kehler, J., Greco, M., Martino, V., Pachiappan, M., Yokoe, H., Chen, A., Yang, M., Jessee, J., Gotte, M., Milanesi, L., Albertini, A., Bellipanni, G., Zucchi, I., Reinbold, R. A. and Giordano, A. (2016), RNA-Generated and Gene-Edited Induced Pluripotent Stem Cells for Disease Modeling and Therapy. J. Cell. Physiol. 2016 Sep 15. doi: 10.1002/jcp.25597. [Epub ahead of print]
|Recommended Use||Feeder layer for mRNA-based cellular reprograming|
|Cell Source||Human Newborn Foreskin Tissue|
|Product Unit||Cryopreserved vial|
|Size||4-5 million cells/vial|
|Storage||Vapor phase of liquid nitrogen|
|Validation Testing||Tested for attachment, mitotic arrest, heath and viability in culture during repeated transfections for the duration of the reprogramming process|
|Quality Testing||Tested for high efficient maintenance of human pluripotent cells, sterility, human pathogens, mycoplasma, post thaw viability|
|Misc.||Not intended for human or animal diagnostic or therapeutic uses|
NuFF-RQ™ Human Fibroblasts
- Product Code: GSC-3006G
- Availability: In Stock