• Human FGF-2 Recombinant Protein

Human FGF-2 Recombinant Protein

Human Recombinant Basic Fibroblast Growth Factor is a member of the fibroblast growth factor (FGF) family. FGF (often referred to as bFGF or FGF-2) family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.

Human Fibroblast Growth Factor-2 (FGF-2) is a recombinant protein produced in  the endosperm of barley grain (Hordeum vulgare). It is a single, non-glycosylated, polypeptide chain containing 146 amino acids, plus a 10 amino acid tag at the N-terminus resulting in a 17.8 kDa protein. FGF-2 is purified by proprietary chromatographic techniques.

Applications

Pluripotent human stem cell (iPSC & ESC) culturing: Human FGF-2 also promotes long-term maintenance and propagation of undifferentiated human embryonic and induced pluripotent stem cells.

Culturing of neural stem cells-human, rat & mouse: FGF-2 is a necessary mitogen for the proliferation of neural stem cells and their maintenance of multipotency.

Activity

Activity is measured using a cell proliferation assay with NIH-3T3 cells. Activity is <0.2 ng/mL.

Purity and Identity

> 95%, by SDS-PAGE analysis with Coomassie blue staining. Identity is confirmed by Western blot analysis using protein specific antibody, ab10420 from Abcam.

Technical Data

Publications

Information
Recommended Use Culturing of human ESC or iPSC; Neural Stem Cells
Product Unit Vial
Size 50 µg
Species/Strain Produced in endosperm of barley grain (Hordeum vulgare).
PluriQ™ Tested on human and mouse pluripotent stem cells over multiple passages
Purity > 95% by SDS-PAGE gel analysis; Carrier-free
Biological Activity <0.2 ng/mL as measured using a cell proliferation assay using 3T3 cells.
Molecular Weight 17.8 kDa
Storage Store at -20°C. Lyophilized protein is stable at room temperature for a few weeks, however the best storage is -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Quality Testing Purity, Activity and Sterility
Shipping Room temperature
Other For Research Use Only - Not intended for human or animal diagnostic or therapeutic uses

Human FGF-2 Recombinant Protein

  • Product Code: GSR-2001
  • Availability: In Stock
  • $154.80


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