Primary Rat Neural Stem Cells - 2M

Primary neural stem cells isolated from the cortical neuroepithelium of the fetal rat (embryonic day 14) at P0. They are reproducible and multipotent. After plating, the cells are ready for expansion or differentiation into neurons, astrocytes and oligodendrocytes.  They can be transfected, transplanted or differentiated into electrically active neurons. We take care of everything from dissection and isolation to cryopreservation. Plus, we perform extensive quality control and performance testing on every batch.

  • Doubling time: 18 hours
  • Medium renewal: Every two days add 10 ng/mL FGF-2  every day.
  • Complete growth medium: N2 / DMEM-F12 plus 10 ng/mL FGF-2
  • Subculture frequency: Every 3–4 days
  • Cryopreservation medium: N2 / DMEM-F12  plus 10% DMSO.

Undirected differentiation to neurons, astrocytes and oligodendrocytes can be achieved by the withdrawal of the mitogen basic fibroblast growth factor (bFGF).

Figure 1. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, MAP2(red), and the astrocyte marker, GFAP (green). The nuclei are stained with DAPI (blue).

 

Figure 2. Differentiated Rat Neural Stem Cells, co-stained for the neuronal marker, Tuß3(left, green), and the astrocyte marker, GFAP (right, green). The nuclei in both images are stained with DAPI (blue).

Technical Data

References

Slotkin TA, Skavicus S, Card J, Levin ED, Seidler FJ. Diverse Neurotoxicants Target the Differentiation of Embryonic Neural Stem Cells into Neuronal and Glial Phenotypes. Toxicology. 2016 Nov 2.

Information
Recommended Use Neural differentiation
Type Rat Sprague-Dawley E14 cortex
Product Unit Cryopreserved vial
Size 1 vial, >1 million cells
Storage Liquid nitrogen
Quality Testing Tested for healthy undifferentiated growth, sterility, Mycoplasma, post-thaw viability
Shipping Dry ice

Primary Rat Neural Stem Cells

  • Product Code: GSC-8010
  • Availability: In Stock
  • $539.96


Related Products

HIP™  Neural Stem Cells, (BC1 Line)

HIP™ Neural Stem Cells, (BC1 Line)

HIP™ Neural Stem Cells, (BC1 Line)These  HIP™ Neural Stem Cells (NSCs) are multipotent cells de..

$754.69

GS22™ Neural Supplement (50X)

GS22™ Neural Supplement (50X)

GS22™ Neural Supplement A serum‐free neural media supplement specifically optimized for the pro..

$91.80

Primary Rat Cortical Neurons

Primary Rat Cortical Neurons

Primary Rat Cortical NeuronsThe cerebral cortex plays an important role in memory, higher thinking, ..

Call/See Options for Price

N2 Supplement (100X)

N2 Supplement (100X)

N2 Supplement A chemically defined, animal-free, supplement used for expansion of undifferentiated ..

$75.37

Tags: primary, rat, neural, stem, cells, human, rodent