A Neuronal Basal Medium for Maximum Growth

A serum-free basal medium optimized for maximum growth and survival of primary and iPSC-derived neural cells in culture, and based on the previously published formulation by Brewer et al.1 Intended to be supplemented with the serum-free GS21™ Neural Supplement (GSM-3100) along with other standard neuronal growth factor supplements, NeuralQ™ Basal Medium shows superior performance in the overall cell health and viability of neurons during short and long-term culture.

Supports Healthy Primary Neurons in Culture

NeuralQ Basal Medium Supports Primary Neurons in Culture 4 Weeks

Image 1.  Healthy, maturing primary rat neurons in culture - NeuralQ® Basal Medium supports maximum viability and healthy of primary rat neurons during short and longer-term culture.

  

Image 2. NeuralQ® supports development of mature neurons - Above images of primary rat neurons (Map2ab, green) cultured 15 days in NeuralQ® Basal Medium shows healthy, mature neurons in culture.

Outperforms Neurobasal® Medium in Long-Term Cultures

   

Image 3. NeuralQ® outperforms Neurobasal® Medium - Above images of primary rat neurons (Map2ab, green) were cultured 3 weeks in NeuralQ® Basal Medium (panel A) or Neurobasal (panel B) and supplemented with GS21™ Neural Supplement. The above data shows NeuralQ® Basal Medium significantly outperforms Neurobasal in supporting neurons growth (green) during long term culture. GFAP staining (red) and Hoechst (blue) label astrocytes and nuclei, respectively.

Significantly Better Support of Primary Mouse Cortical Neurons

  
Image 4. GS21 Supplemented NeuralQ® Basal Medium for Superior Performance in Mouse Neurons - Primary Mouse Cortical Neurons (ThermoFisher,  Cat No. A15585) were cultured in GS21 supplemented NeuralQ Basal Medium (left image) or B27® Supplemented Neurobasal (right image) for two weeks. Cells were then fixed and stained with Map2 (green) to label maturing neurons. Above representative images show NeuralQ/G21 better supports mouse neuronal maturation than does Neurobasal/B27.


Reference: Brewer, G.J., Torricelli, J.R., Evege, E.K. and Price, P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, A new serum-free medium combination. J. Neurosci. Res.35: 567-576 (1993).

Technical Data

Publications


NeuralQ & GS21 are registered Molecular Transfer, Inc trademark products

Information
Recommended Use Basal medium for maximum growth and survival of primary and iPS-derived neurons in culture.
Product Unit Bottle, sterile-filtered
Size 500mL
Concentration 1X
Storage Refrigerate at 4°C
Quality Testing Sterility, bacterial and fungal testing
Shipping Room temperature
Other Not intended for human or animal diagnostic or therapeutic uses

NeuralQ® Basal Medium (1X)

  • Product Code: GSM-9420
  • Availability: In Stock
  • $58.93


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Tags: neuralq, basal, medium, (1x), media, supplements neuralq®