A chemically defined, animal-free, supplement used for expansion of undifferentiated neural stem and progenitor cells in culture. N2 Supplement can also be used for maintenance of post-mitotic primary neurons in culture. N2 Supplement is intended for use with DMEM/F12 medium. While N2 can be used to grow primary neurons, our data indicates that NeuralQ® Basal Medium (GSM-9420) with GS21™ Neural Supplement (GSM-3100) is better suited for this application.
Supports Healthy Neural Stem Cells in Culture
Figure 1. N2 Supplement supports healthy growth of proliferating rat primary neural stem cells in culture over multiple passages. Primary Rat Neural Stem Cells (GSC-8010) were cultured in DMEM/F12 with N2 supplement. After 3 passages, cells were co-labeled with Sox2 (green) and Nestin (red).
||Concentration (100X supplement)
|Recombinant Human Insulin
- Bottenstein JE, Sato GH. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci U S A. 1979 Jan;76(1):514-7.
- Slotkin TA, Skavicus S, Card J, Levin ED, Seidler FJ. Diverse Neurotoxicants Target the Differentiation of Embryonic Neural Stem Cells into Neuronal and Glial Phenotypes. Toxicology. 2016 Nov 2.
- Slotkin TA, Skavicus S, Card J, Di Giulio RT, Seidler FJ. In vitro models reveal differences in the developmental neurotoxicity of an environmental polycylic aromatic hydrocarbon mixture compared to benzo [a] pyrene: Neuronotypic PC12 Cells and embryonic neural stem cells. Toxicology. 2017 Feb 15;377:49-56.
|Recommended Use||Expansion of undifferentiated neural stem and progenitor cells|
|Product Unit||Bottle, sterile-filtered|
|Size||5mL, 100X Concentration|
|Quality Testing||Tested for neural cell expansion & undifferentiation, Sterility, Mycoplasma, pH, endotoxin, osmolarity, and other|
|Other||FOR RESEARCH USE ONLY - Not intended for human or animal diagnostic or therapeutic uses|
N2 Supplement (100X)
- Product Code: GSM-3300
- Availability: In Stock