Effect of DNA Amount on Transfection-Related Cellular Toxicity -NewTransfection is the process of introducing new nucleic acids into a cell. Quite often, the thought process in transfection is “more is better” – that is, the goal is to achieve the highest possible protein expression post-transfection. However, cells can be stressed by over-expression of specific proteins, especially when over-expression takes over the protein production machinery at the expense of producing other necessary proteins for the cell or by an innate immune response to CpG islands in DNA. In this application note, we examine the effect of DNA amount on transfection-related cellular toxicity.
Titration of mRNA Delivery: An Approach for Efficient Transfection with Modified or Unmodified mRNA
The ability to transfect a population of cells at high efficiency with mRNA offers many advantages over DNA transfection. In this application note, we address the differences between modified and unmodified mRNA on transfection efficiency and toxicity in a variety of cells, including cells lines that are considered difficult to transfect.
Optimal Conditions for Embryonic and Neural Stem Cell Culture
Growing stem cells in culture is a delicate balancing act between providing an optimal environment for maintaining pluripotency and guiding development down specific pathways when desirable. This article discusses several exciting new developments in embryonic and neural stem cell culture techniques.
Impact of media additives on stem cell behavior in culture
Stem cells are particularly sensitive to media composition.Even trace amounts of contaminants, such as endotoxin, can lead to altered biological responses in stem cells This paper examines key information regarding the impact of media additive quality on stem cell characteristics in vitro.
Tips for maintaining primary neuronal cell cultures long-term
Growing primary neurons long term in vitro can sometimes prove to be a daunting task especially when your cells are highly susceptible to infection and hyperosmolarity due to medium evaporation. Here we present 5 cell culture tips for maintaining primary neurons in culture long-term.
Cryopreserved neurons - a better alternative?
Primary rat and mouse neuronal cultures are widely used by researchers to study basic physiological properties of neurons. Traditionally, there has been the belief that frozen neurons are not as "good" as fresh neurons and for many laboratories, this meant dissecting rats to use fresh neurons prior to every experiment. Here we discuss why using cyropreserved neurons may be a much better alternative.
Human induced pluripotent neural stem cells
The interest in Induced Pluripotent Stem Cells (iPSC) has intensified over the years and proves to be promising in its ability to impact human health. The field is exploding with new methods for generating iPS cells and there are still questions about their performance in vitro compared to rodent neurons. What are the advantages to using iPS cells vs. rodent neurons? Can these neurons derived from iPS cells differentiate and function in vitro in the same ways that rodent neurons do? Can they be used in a HTS system to improve the success rate of drug discovery?
Using the Right Combination of Media, Supplements and Neural Substrates
Growing and maintaining primary neurons long-term in culture requires excellent cell-culture handling skills along with an optimal combination of media, serum-free supplements and substrates. Paying close attention to these culture conditions is important to ensuring your primary neurons’ survival. Here we answer some of your questions about culturing healthy primary neurons, and offer tips to make the process as efficient as possible.
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