• GS22 Neural Supplement (50X)

GS22™ Neural Supplement

 A serum‐free neural media supplement specifically optimized for the propagation and long‐term viability of proliferative neural cells such as human iPS-derived neural stem cells or rat primary oligodendrocyte precursors. GS22™ Supplement is intended to be used with NeuralX™ Basal Medium (GSM-9320).

  • Improves long‐term viability of proliferative neural cells. 
  • Supports more efficient and higher yields of oligodendrocyte precursors.
  • Serum-free, animal-free for minimal risk of introducing adventitious agents into the cell.

Supports Healthy Neural Stem Cells in Culture

 BC1 NSC grown in NeuralX with GS22 passage 2 

Figure 1.  GS22™ Neural Supplement supports healthy growth of proliferating human iPSC-derived neural stem cells in culture over multiple passages. HIP™ Human iPSC-derived pluripotent neural stem cells (BC1 line) were maintained in NeuralX™ NSC Medium and GS22™ Supplement for 4 days before passaging and continuing culture for another 4 days. 

Supports Growth & Long-term Survival in Oligodendrocyte Precursors

Rat primary oligodendrocyte precursor in GS22 

Figure 2. Rat primary oligodendrocyte precursors (GRPs) exhibit excellent growth, survival and health when grown in GS22™ Neural Supplement and NeuralX™ NSC Medium . After rat GRPs were grown for 3 passages in  GS22™ supplement, antibody labeling indicated the cells were still primarily undifferentiated GRPs, as indicated by labeling with A2B5 (green) and DAPI (blue). 

Optimal Differentiation into Healthy, Mature Oligodendrocytes

mature oligodendrocytes mature oligodendrocytes 

Figure 3. Rat primary oligodendrocyte precursors (GRPs) differentiate into healthy, mature oligodendrocytes. After growing rat GRPs for 3 passages in GS22™ Supplement and NeuralX™NSC Medium cells were switched to NeuralQ™ Basal Medium supplemented with GS21™ Neural Supplement (GSM-3100) . Antibody labeling revealed a significant number of mature, differentiated oligodendrocytes, as indicated by labeling with GalC (green, left panel) and MBP (green, right panel). Total cells are shown using DAPI (blue, both panels)

Technical Data

Publications

  • Barratt, H. E., Budnick, H. C., Parra, R., Lolley, R. J., Perry, C. N., & Nesic, O. (2016). Tamoxifen promotes differentiation of oligodendrocyte progenitors in vitro. Neuroscience.
Information
Recommended Use Propagation and long‐term viability of proliferative neural cells
Product Unit Bottle, sterile-filtered
Size 10mL, 50X Concentration
Storage -20°C
Quality Testing Tested for neuronal cell growth, Sterility, Mycoplasma, pH, endotoxin, osmolarity, and other
Shipping Dry Ice
Other FOR RESEARCH USE ONLY - Not intended for human or animal diagnostic or therapeutic uses

GS22™ Neural Supplement (50X)

  • Product Code: GSM-3200
  • Availability: In Stock
  • $91.80


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Tags: neural stem cells, nsc, supplement, (50x), media, supplements gs22 gs22™