• GS21 Neural Supplement (50X)

GS21™ Neural Supplement

For Maximum Survival & Neuronal Cell Growth

GS21™ is the next generation serum-free neural media supplement, based on the formulation of NS21 Supplement, designed to improve the overall growth and performance of primary neurons1. GS21™ is specifically optimized for the maturation and long-term viability of primary rat and mouse neurons in culture, without the need for co-culture with astrocyte feeder cells.

  • Significantly improves long-term viability of  primary and iPSC-derived neurons in vitro.
  • Enhances neurite outgrowth of primary neurons in culture.
  • Supports growth and maintenance of primary neurons at low or high cell density plating.

Significantly Higher Neuron Viability

GS21 improved neuronal viability

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Fig.1 - Higher neuron viability after 2 & 4 weeks in culture - Primary Rat Cortical Neurons (GSC-8220) cultured in GS21™ supplemented culture media showed significantly higher neuron viability in culture, as compared to cells cultured using 3 competitor supplements. Neurons plated in 96-well plates were labeled with MAP2 (green), and DAPI (blue) to denote nuclei. 

Rat Cortical Neurons cultured in GS21™

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Fig.2 - GS21™ Supplement supports 6-week cultured neurons - Primary Rat Cortical Neurons cultured in GS21 supplemented culture media remain viable after 6 weeks in culture. The above image shows healthy, mature primary neurons identified by MAP2 staining (green). Cells were imaged on a BD Pathway™ Bioimager after 6 weeks in culture. Images were acquired at 10x and 20x (inset) magnifications.

Improved Neurite Outgrowth


Neurons cultured in GS21™

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Fig.3 - Greater neurite outgrowth after 1, 2 and 4 weeks in culture - Primary Neurons were cultured  in GS21™ supplemented neural base media. MAP2 labeling (top, left) was used to determine boundaries of the individual neuron cell bodies and associated neurites (colors are randomly assigned by the software). GS21 supplemented cultures showed significantly greater neurite lengths (top) and number of neurite segments (bottom) in culture, as compared to cultures supplemented with competitor supplement: neurite lengths, 1 week, (p=0.022), 2 week (p=0.017) and 4 week (p=0.023); and number of neurite segments (bottom) in 1 week,(p=0.036), 2 week (p=0.041) and 4 week (p=0.010).  Images were acquired using a 10x objective (0.4 NA). Data from n=4 wells is shown as mean±SEM.

Technical Data

Y, Stevens B, Chang J, Milbrandt J, Barres BA, Hell JW. NS21:re-defined and Modified Supplement B27 for Neuronal Cultures. J Neurosci Methods, 171:(2) 239-0247, 2008.

Publications

Information
Recommended Use Primary rat and mouse neuronal cell culture.
Product Unit Bottle, sterile-filtered
Size 10mL, 50X Concentration
Storage -20°C
Quality Testing Tested for neuronal cell growth, Sterility, Mycoplasma, pH, endotoxin, osmolarity, and other
Shipping Dry Ice
Other FOR RESEARCH USE ONLY - Not intended for human or animal diagnostic or therapeutic uses

GS21™ Neural Supplement (50X)

  • Product Code: GSM-3100
  • Availability: In Stock
  • $85.00


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