EditPro™ Transfection Reagent has been optimized to deliver Cas9 mRNA or RNP with the associated guide RNA, into a wide range of adherent cell types. In addition, ssDNA constructs or single-stranded oligodeoxynucleotides (ssODN) can be mixed with the Cas9mRNA/gRNA or Cas9 protein/gRNA to promote homology-directed repair (HDR) to introduce targeted substitutions. It is well suited for co-transfection delivery of gene editing tools, therefore, into many primary cells and cell lines.
High Efficiency Cas9 mRNA Delivery
Below we demonstrate the efficiency of transfection of Cas9-encoding modified mRNA followed by detection with anti-Cas9 antibodies demonstrating high efficiency transfection and expression of the Cas9.
Cas9-mediated INDEL Formation
The following data in HeLa cells demonstrates successful gene editing with EditPro™ via co-transfection of either Cas9 mRNA or Cas9 protein with the associated guide RNAs as indicated by the formation of INDEL bands.
Figure 2. Indel analysis following transfection with EditPro™ Transfection Reagent. HeLa cells were transfected using 1 or 2 µL of EditPro™ Transfection Reagent as indicated with Cas9 mRNA (modified)/ gBlock® U6sgRNA GAPDH oligo/ GFP mRNA (modified) or Cas9 protein/ gBlock® U6sgRNA GAPDH oligo/ GFP mRNA (modified). Genome-modification was analyzed using the T7Endo 1 assay.
- High Efficiency – delivering Cas9 RNA and/or Cas9 protein for gene editing and DNA for homology-directed repair.
- Low toxicity
- Easy to use – no special equipment required
- Cost Effective – effective at low amounts of reagent resulting in low cost/well
|Recommended Use||Transfection of mRNA, protein (RNP) for genome editing into adherent primary cells and cell lines.|
|Cell Type||Adherent primary cells and cell lines such as fibroblasts, endothelial cells, C2C12, HeLa, HEK293, etc.|
|Classification||Defined, animal origin-free.|
|Sample Type||RNA and/or protein, ssDNA, ssODN|
|Storage||Store refrigerated at 2-8°C. Do Not Freeze.|
|Other||For Research Use Only - Not intended for human or animal diagnostic or therapeutic uses.|
EditPro™ Transfection Reagent
- Product Code: GST-2184
- Availability: In Stock