Stem Cell Biology / Stem Cell Culture
Stem cell culture presents unique challenges as researchers create conditions to either prevent cell development, or direct development along specific pathways. Pluripotent stem cells are uncommitted cells that have not yet started down a specific developmental pathway. The most common pluripotent stem cells studies in the lab are embryonic stem (ES) cells, derived from the inner cell mass of embryos originally created for in vitro fertilization that were donated to research when they were no longer needed. Keeping ES cells in an undifferentiated state in the lab requires the presence of a number of extrinsic factors, such as growth factors added to the medium and/or provided by growth with a feeder layer of cells.
One challenge of studying stem cell biology is their resistance to transfection using more commonly used transfection reagents. Transfection is an incredibly useful tool in stem cell biology – it is used to reprogram somatic cells to produce induce pluripotent stem cells (iPSCs), and engineer stem cells for specific therapeutic purposes. However, stem cells are notoriously difficult to transfect. Even neural stem cells, which are still proliferating, are difficult to transfect even with virus-based vectors. This sensitivity led Fred Gage and Sally Temple (2013) to include “better nonviral transfection methods” in order to “accelerate and reduce the cost of answering many questions about” neural stem cells on their wish list of technologies to enable critical advances in neural stem cell research.
MTI-GlobalStem offers stem cells, feeder cells, cell culture media and growth factors to keep cells in an undifferentiated state, or direct cells towards a specific developmental pathway. MTI-GlobalStem specializes in developing proprietary nanoparticles described as Nucleic Acid Transporters for optimized transfection with minimal cell toxicity in embryonic and adult stem cells. Easily transfect stem cells without inhibiting growth and viability. We offer a broad portfolio of stem cell characterization tools and services to ensure your experimental results so you can present your findings with confidence.
- Gage, F. H. and Temple, S. (2013). Neural Stem Cells: Generating and Regenerating the Brain. Neuron, 80(3), 588-601.
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