Mouse Embryo Development
Preimplantation-stage mouse embryos have been used since the mid-1950s for a variety of biological applications. In recent years, mouse embryos have been used for the creation of genetically modified embryonic stem cells to create transgenic and knockout mice, as well as used in the mouse embryo assay (MEA) for assessing toxicity.
The creation of transgenic mice, including knockout mice, requires careful handling of mouse embryos. In particular, cell culture media quality can affect the reproducibility of mouse embryo culture. MTI-GlobalStem offers a variety of mouse embryo media for the collection, manipulation and transfer of mouse embryos.
Some references for methods for handling mouse embryos include:
- Lawitts JA, Biggers JD, J Reprod Fertil Optimization of mouse embryo culture media using simplex methods March 1, 1991 91 543-556
- Hogan B, Costantini F, Beddington R, Lacy E, Manipulating the Mouse Embryo: a Laboratory Manual, Second Edition 1994 Cold Spring Harbor Press.