A defined, feeder-free maintenance medium for pluripotent stem cell culture

PluriQ™ G9 Maintenance Medium is a highly defined feeder-free cell culture medium that provides a xeno-free growth environment for the feeder-free maintenance and expansion of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC).  The PluriQ™ G9 Maintenance Medium  contains all of the essential components ( base  medium and supplements) required to keep cells alive, growing and healthy after reprogramming, and backed by the stringent PluriQ™ quality standards that set MTI-GlobalStem apart from competitors. Each lot of medium is pre-qualified to ensure consistent cell culture performance.

Easily maintain reprogrammed pluripotent cells in culture

The PluriQ™ G9 Maintenance Medium kit creates the ideal cell culture environment for expanding and maintaining human ES and iPS cells in culture.  Stem cells are seamlessly transitioned to the PluriQ™ G9 Medium for hESC/hIPSC Maintenance following reprogramming in the PluriQ™ G9 Medium system for reprogramming (GSK-9001). PluriQ G9™ is compatible with vessels coated with vitronectin, Matrigel®, Geltrex®, or Laminin-521.


Figure 1.  PluriQ™ G9 feeder-free cell culture medium supports maintenance in culture of human pluripotent stem cells. Human  iPSCs shown here were passaged onto vitronectin in complete PluriQ™ G9 medium and expanded for 4 days. Multiple passages produced similar results (data not shown).


Figure 2. PluriQ™ G9 feeder-free cell maintenance medium  maintains undifferentiated human iPSC NCRM-1 cells in culture. Cells stained with SSES4 (green) and OCT4 (red) are co-stained with Hoescht (blue).

Components:

  • PluriQ™ G9 Basal Medium (500 ml) – GSM-9001
  • PluriQ™ G9 Supplement (50x, 10 ml) – GSR-9001
  • Human FGF2 (bFGF, 10 µg) – GSR-2001-10
  • Human TGF-β (1 µg/ml) – GSR-2051 

PluriQ™ Tested

Our PluriQ™ reagents is tested for quality, purity and the effective maintenance of pluripotent stem cells over multiple passages to ensure health, morphology and undifferentiation. 
Each lot of PluriQ™ Medium undergoes testing for:
  • Sterility
  • Mycoplasma
  • pH
  • Osmolarity

Technical Data

  • Product Protocol

  • Publications

    • Kehler, J., Greco, M., Martino, V., Pachiappan, M., Yokoe, H., Chen, A., Yang, M., Jessee, J., Gotte, M., Milanesi, L., Albertini, A., Bellipanni, G., Zucchi, I., Reinbold, R. A. and Giordano, A. (2016), RNA-Generated and Gene-Edited Induced Pluripotent Stem Cells for Disease Modeling and Therapy. J. Cell. Physiol. 2016 Sep 15. doi: 10.1002/jcp.25597. [Epub ahead of print]
    Information
    Recommended Use Xeno-free maintenance of human pluripotent stem cells in culture after reprogramming
    Size 1 Kit Contains: ● PluriQ™ G9 Basal Medium - 500ml, (GSM-9001) ● PluriQ™ G9 Supplement (50X), -10ml, (GSR-9001) ● Human FGF-2 (10µg/0.5ml), (GSR-2001-10) ● Human TGF-β1 (1µg/ml) - 500µl, (GSR-2051)
    PluriQ™ Tested on human pluripotent stem cells
    Storage Store refrigerated at 2-8°C: ● PluriQ™ G9 Basal Medium Store -20°C (non frost-free freezer): ● PluriQ™ G9 Supplement ● Human FGF-2 ● Human TGF-β1
    Quality Testing Tested for healthy undifferentiated growth, sterility, mycoplasma, post-thaw viability
    Shipping At room temperature: ● PluriQ™ G9 Basal Medium On dry Ice: ● PluriQ™ G9 Supplement, ● Human FGF-2 ● Human TGF-β1

    PluriQ™ G9 Maintenance Medium

    • Product Code: GSK-9001
    • Availability: In Stock
    • $194.25


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    Tags: pluriq, feeder-free, xeno-free, medium, media, ipsc, pluriq™, maintenance kit, g9 for, stem, cells