• mRNA-In Transfection Reagent

mRNA-In® 

A Universal Transfection Reagent for High Efficiency mRNA Delivery

mRNA-In® Transfection Reagent was developed by members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000 and specifically formulated for in vitro mRNA delivery. Maximum RNA delivery is achieved with mRNA-In® using lower amounts of mRNA than other reagents, reducing experimental costs and significantly lowering toxicity. Across a wide range of cell types, mRNA-In™ has shown to produce exceptionally high transfection efficiency while maintaining optimal cell health and viability.

Features

  • Requires low amounts of RNA - reduce cost and potential off-target toxicity effects with low amounts of RNA requirements.
    •  Transfects a variety of cell lines with as little as 2 pg/cell of mRNA
  • High expression, low toxicity - achieve high mRNA efficiency while maintaining optimal cell health for uncompromised experiment results.
  • Robust, versatile performance - produce efficient transfection across a wide range of cell type, including stem cells and primary cells.

Applications

mRNA-In® Transfection Reagent is ideal for mRNA delivery for various applications, such as protein expression and iPSC-reprogramming. Data below also shows mRNA-In® significantly outperforms competitor reagents, typically achieving 90% or greater transfection efficiency across a wide range of cell types.

Delivers Maximum Transfection Efficiency using Low Amounts of mRNA

Data below show commonly used cell types transfected with mRNA-In® Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with various amounts of mRNA-In® in a total of 50µl of OptiMEM®.  For each experiment mRNA /mRNA-In™ complexes were added to cells, mixed, and incubated at 37ºC in 5% CO2.  Cells were observed 24 hours following transfection. 


Higher  Expression, Significantly Outperforms the Leading Competitors

Below, SH-SY5Y (neuroblastoma cells), HDF (human dermal fibroblasts), HUVEC (human umbilical vein endothelial cells), HeLa and adipose-derived Mesenchymal Stem Cells (MSCs) were transfected with mRNA-In™ or MessengerMAX™ (Life Technologies) using low amounts of mRNA (100ng). Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with the various amounts of mRNA-In® (see chart legends). RNA/reagent complexes were added to the cells, mixed, and incubated at 37ºC in 5% CO2 overnight.  Cells were analyzed by a fluorescence plate reader and microscopy 24 hours post-transfection. Error bars represent the standard deviation of triplicate wells. The data below shows mRNA-In™ provides significantly higher transfection efficiency than the competitor reagent.


Technical Data

  • "Quick", Easy-to-follow protocol  - Updated 1/7/16
  • Product sheet

  • Posters

    Information
    Cell Source Formulated for most commonly used cell lines and primary cells.
    Classification Defined, animal origin-free
    Size 0.1 ml, 1 ml, 5x1 ml or 10x1 ml
    Sample Type mRNA
    Shipping Ships at room temeperature; Store at 4C, DO NOT FREEZE
    Other FOR RESEARCH USE ONLY - Not intended for animal or human therapeutic use or diagnostic use

    mRNA-In® Transfection Reagent

    • Product Code: 73740
    • Availability: In Stock
    • Call/See Options for Price


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    Tags: mrna-in, transfection, reagent, general-purpose, reagents, 73741, 73742, 73743 mrna-in®