Gene Expression / Transfection
Major advances have been made in the design and development of synthetic gene delivery vectors, including cationic polymers and lipids. MTI-GlobalStem scientists specialize in developing Nucleic Acid Transporters (NATs), which are nanoparticles made up of distinct components that are used in combination to deliver nucleic acids into cells. Utilizing our extensive knowledge and expertise in this area, our goal is to create synthetic nanoparticles that have the molecular delivery efficiency of viruses while providing a safer, easier-to use, and more cost-effective transfection system alternative.
Transfection: An essential method for researchers
Choosing a good transfection system - Effective transfection reagents provide consistently high transfection efficiencies across a diverse array of cell types, have low toxicities so as not to negatively impact downstream experiments, are simple-to-use, and are budget friendly. When selecting a transfection reagent, productivity and data confidence are also important.
A balance between transfection efficiency and toxicity - The transfection reagent:DNA complex can be toxic to cells. Most protocols recommend that cells recover ≥16h post-transfection to allow for the overall metabolic activity of the cells to return to near normal. Our reagents show less residual toxic effects even at 48h post-transfection for many cell lines. Lower toxicity provides greater confidence in results being due to biological differences rather than reagent artifacts. This is even more important when interpreting results from particular cell types like stem cells, where the population may not be large, and where the cells are difficult to obtain and/or are more sensitive to the toxic effects of transfection. When planning your transfection-based experiments, it is important to optimize the lipid:DNA ratios so that you may balance transfection efficiencies with toxicity. The dose response experiments show that each cell line may have different optimal lipid:DNA ratios. Certainly, you may use the given volumes and amounts as initial guides, but for optimal results, we recommend that you initially perform similar experiments for each cell line being used.
DNA vs mRNA Transfection - MTI-GlobalStem offers a family of DNA and mRNA transfection reagents that balance high transfection and expression levels with low toxicity across a range of neural, stem and primary cells, and a wide variety of cell lines. Developed by the lead members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000, our reagents are formulations of chemically-defined proprietary compounds of animal-free origin, and are routinely tested on a wide range of cell types designed to require minimal optimization to achieve superior gene-delivery and expression results.
Advances in genome editing have expanded the need for delivery methods with the ability to transfect large DNA constructs or a combination of DNA, RNA and/or protein. DNA-In CRISPR® is optimized for intracellular transfection of CRISPR/Cas9 all-in-one vectors into primary cells and other cell types for Genome Editing applications. EditPro™ Stem was developed to deliver DNA, RNA and/or protein (RNP) efficiently into pluripotent and neural stem cells to maximize gene editing by a variety of methods. The PluriQ™ G9™ Gene Editing System combines optimized media for culturing human pluripotent stem cells, vitronectin matrix, versene for passaging and EditPro™ Stem in order to optimize all the conditions for maximum performance. EditPro™ transfection reagent was optimized for the delivery of Cas9 mRNA or protein (RNP) and the related guide RNA for CRISPR gene editing in a range of adherent primary cells and cell lines. . In addition to supporting high-efficiency indel formation, ssDNA constructs or single-stranded oligodeoxynucleotides (ssODN) can be mixed with the Cas9mRNA/gRNA or Cas9 protein/gRNA to promote homology-directed repair (HDR) to introduce targeted substitutions..
Neural Cell Transfection - Our neuron-specific transfection reagents are formulated for high efficiency DNA and mRNA delivery in neurons with low cytotoxicity. Learn more below about DNA-In® Neuro and mRNA-In® Neuro Transfection Reagents.
Transfection Reagents for Commonly Used Cell Lines - Modifying gene expression is more difficult in some cell types than others. Scientists have developed a range of tools to deliver nucleic acids to different types of cells, with varying degrees of success. Our cell-specific transfection reagents included reagents that have been optimized and validated for use in a number of commonly used cell types. For other cells, our DNA-In® and mRNA-In® "Universal" reagents are available for researchers to optimize in their own labs with our expert assistance.
For more information on all our Transfection Reagents