NEW DNA-In® CRISPR Transfection Reagent  

Specifically formulated for CRISPR/Cas9 transfection reagent

DNA-In® CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors  into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells.  Below, a Cas9-GFP plasmid was delivered into primary fibroblasts, muscles cells, keratinocytes and others primary cells and commonly used cell types with high efficiency and low toxicity.

Figure 1. DNA-In® CRISPR delivers Cas9-GFP expression vector with maximum efficiency  - Human primary fibroblasts, human primary keratinocytes, primary human  endothelial cells (HuVEC), human skeletal muscle cells, HeLa cells  and C2C12  mouse myoblasts were plated in 24-well plates in complete medium without antibiotics at the optimal plating density for each cell type to yield 50-70% confluency at the time of transfection. Cells were transfected with the pSpCas9(BB) -2A-GFP pasmid  (9.3kb )(source: Addgene), using DNA-In® CRISPR reagent and incubated overnight at 37°C. Cells were then fixed  and stained with anti-GFP and anti-Cas9 antibodies to better observe the transfection efficiency. 

Learn about High Efficiency CRISPR-Cas9 Delivery in Stem Cells

Highly efficient Cas9 delivery in primary cells

dna-in crispr transfection of fibroblasts lipofectamine 2000 transfection of fibroblasts lipofectamine 3000 transfection of fibroblasts

 Human dermal primary fibroblasts (above) were plated  to give ~70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

dna-in crispr transfection of keratinocytes lipofectamine 2000 transfection of keratinocytes lipofectamine 3000 transfection of keratinocytes

 Human primary keratinocytes (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K , L3K). 

dna-in crispr transfection of c2c12 lipofectamine 2000 transfection of c2c12 lipofectamine 3000 transfection of c2c12

 C2C12 mouse myoblasts  (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

dna-in crispr transfection of skmc lipofectamine 2000 transfection of skmc lipofectamine 3000 transfection of skmc

 Human skeletal muscle cells (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

Features

  • Optimized for large plasmid DNA containing Cas9/GFP/ guideRNA ( all-in-one).
  • High efficiency delivery of Cas9 expression vectors in hard-to-transfect primary cells.

Technical Data


DNA-In® is registered trademarks of Molecular Transfer, Inc.

Lipofectamine®, Opti-MEM® are registered trademarks of Thermo Fisher Scientific Inc.

Information
Recommended Use Large plasmid transfection of CRISPR/Cas9 vectors into a range of adherent cell types.
Cell Type Formulated specifically for CRISPR/Cas9 all-in-one vector transfection into a wide range of adherent cells.
Classification Defined, animal origin-free
Size 0.1 ml, 1 ml, 5x1 ml and 10x1 ml
Sample Type Plasmid DNA
Serum Compatibility Yes
Storage Store refrigerated at 2-8°C. Do Not Freeze.
Shipping Ships at room temperature; Store at 4°C ; DO NOT FREEZE
Other FOR RESEARCH USE ONLY - Not intended for any animal or human therapeutic or diagnostic use.

DNA-In® CRISPR Transfection Reagent

  • Product Code: GST-2160
  • Availability: In Stock
  • Call/See Options for Price


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Tags: dna-in, CRISPR, transfection reagent, CRISPR/Cas9, genome-editing, GST-2161, GST-2162, GST-2163 dna-in®, crispr, crispr/cas9, delivery, system technology